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詳細解析！et404毒素試劑盒的檢測原理和步驟
發(fā)布時間： 2023-02-26 點擊次數： 879次et404毒素試劑盒主要是用來檢測毒素的一種經濟、快捷的檢測初篩工具，現在當前市面上主要有毒素B1試劑盒、毒素M1試劑盒、毒素總量試劑盒。它的檢測原理：et404毒素試劑盒采用競爭ELISA，在微孔板上預包被毒素抗原，加入樣本/毒素總量標準品溶液及辣根過氧化物酶標記的毒素總量抗體。樣本或標準品溶液中的毒素與預包被在微孔板上的毒素抗原競爭結合辣根過氧化物酶標記的毒素總量抗體。未結合的酶標抗體在洗滌時被除去，再加入TMB顯色液，讀取吸光值。樣本的吸光值與其所含殘留物毒素總量的含量成負相關。對照標準曲線，即可得出相應殘留物毒素總量的含量。et404毒素試劑盒的檢測步驟：樣品處理：取1ml樣品置于5ml離心管中，向離心管中加2ml提取劑，振搖2～3min后，用離心機以2000r/min，離心5min，取2ml上清液至另一個5ml離心管中，向其中加2ml凈化劑，振搖5min，用離心機以2000r/min離心5min，吸取全部下層至點滴板的一個孔中自然揮干（或加熱將其揮干），備用；樣品檢測：向點滴板上滴加4滴緩沖液，使其充分溶解揮干后的殘留物；取出檢測卡，并做好標記，用袋中吸管吸取點滴板上的全部樣液，滴加到檢測卡的加樣孔中（逐滴滴下），5～10min內觀察結果；結果判斷：陰性：測試線(T)與對照線(C)都出現，表明樣品中毒素的濃度小于0.5μg/kg。陽性：只有對照線(C)，無測試線(T)，表明樣品中毒素的濃度大于或等于0.5μg/kg。無效：對照線(C)和測試線(T)都不出現，或者對照線（C）不出現。
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